Protein crystal structure determination with the crystallophore, a nucleating and phasing agent - Groupe Membrane et pathogènes / Membrane and Pathogens Group (IBS-MP)
Article Dans Une Revue Journal of Applied Crystallography Année : 2019

Protein crystal structure determination with the crystallophore, a nucleating and phasing agent

Résumé

Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches
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hal-02176249 , version 1 (06-01-2025)

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Sylvain Engilberge, Tristan Wagner, Gianluca Santoni, Cécile Breyton, Seigo Shima, et al.. Protein crystal structure determination with the crystallophore, a nucleating and phasing agent. Journal of Applied Crystallography, 2019, 52 (4), pp.722-731. ⟨10.1107/S1600576719006381⟩. ⟨hal-02176249⟩
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