Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem
Résumé
Bidimensional and monodimensional polyacrylamide gel electrophoresis were used to study protein expression from zooplankton collected in thirteen stations of Toulon Bay (NW Mediterranean). In this ecosystem, Little Bay showed higher trace metal concentrations (13.5–23.8 nM for Cu, 0.73–1.24 nM for Pb, 27.8–58.7 nM for Zn) than Large Bay (Cu 2.2–15.6 nM; Pb 0.19–0.78 nM; Zn 9.0–38.8 nM). Trace metals positively correlated (p < 0.05) with expression of four zooplankton proteins (MW in kDa/pI: 25.0/5.6; 48.8/4.1; 38.2/4.4; 38.3/5.8) and with biomass of Oithona nana, predominant copepod in Little Bay. Sequencing by LC–MS/MS putatively provided zooplankton identity of these proteins: they were cytoskeleton actin, except one protein that was the chaperone calreticulin. We suggest that actin and calreticulin could be regarded as zooplankton markers of metal stress and be involved in a possible tolerance of O. nana to contamination, contributing to its development in a marine perturbed ecosystem.
Mots clés
Bidimensional polyacrylamide gel electrophoresis Monodimensional polyacrylamide gel electrophoresis
Liquid chromatography tandem mass spectrometry
Trace metals
Zooplankton
POLYACRYLAMIDE-GEL ELECTROPHORESIS
TOXIN-PRODUCING DINOFLAGELLATE
DISSOLVED ORGANIC-MATTER
PACIFIC SURFACE WATERS
HARMFUL ALGAL BLOOMS
SOUTH CHINA SEA
2-DIMENSIONAL ELECTROPHORESIS
ALEXANDRIUM-TAMARENSE
PROTEOMIC APPROACH
Origine | Fichiers produits par l'(les) auteur(s) |
---|
Loading...